Question 1

Which of the following would not be considered a form of biotechnology?

Accepted Answer

A)   Selective breeding to produce cows with greater milk yield
B)   Pharming
C)   Use of microbes to produce an antibiotic
D)   The synthetic production of nylon using organic chemistry techniques
E)   All of the above are examples of biotechnology.F) A) and B)
G) A) and C)

Question 2

The joining of DNAs from two or more genetic sources DNA is called

Accepted Answer

A)   PCR.
B)   recombinant DNA.
C)   complementary DNA.
D)   DNA fingerprinting.
E)   None of the aboveF) B) and E)
G) A) and B)

Question 3

The DNA from the genome of an organism is chopped into numerous fragments, which then are inserted into vectors that are taken up by host cells. The genetic information that is present in the resulting colonies is called a genomic

Accepted Answer

A)   collection.
B)   library.
C)   museum.
D)   bank.
E)   farm.F) B) and D)
G) D) and E)

Question 4

Libraries constructed from only those genes that are expressed in a given tissue are known as _______ libraries.

Accepted Answer

The answer of Libraries constructed from only those genes that...

Question 5

An enhancer would most likely be used for

Accepted Answer

A)   constructing a gene library.
B)   constructing an expression vector.
C)   performing a restriction digestion.
D)   attempting to "silence" a gene.
E)   constructing a recombinant plasmid.F) C) and E)
G) A) and C)

Question 6

_______ would be added to an expression vector if the researchers wanted to direct the gene product to the nucleus after its production.

Accepted Answer

The answer of _______ would be added to an expression...

Question 7

A biologist would most likely use the technique of electroporation to

Accepted Answer

A)   separate proteins based on size and charge.
B)   knock out a gene.
C)   create antisense DNA.
D)   measure expression levels of a gene.
E)   coax DNA into host cells.F) D) and E)
G) A) and D)

Question 8

Complementary base pairing is important for

Accepted Answer

A)   ligation reactions with blunt-end DNA molecules.
B)   hybridization between DNA and transcription factors.
C)   restriction endonucleases for cutting cell walls.
D)   synthesizing cDNA molecules from mRNA templates.
E)   the transcriptional activation of expression vectors.F) C) and D)
G) B) and E)

Question 9

The production of a hormone in milk from a transgenic cow is an example of _______.

Accepted Answer

The answer of The production of a hormone in milk...

Question 10

Vectors include

Accepted Answer

A)   bacterial plasmids.
B)   viruses.
C)   plant plasmids.
D)   bacteriophage  λ\lambdaλ .
E)   All of the above F) A) and C)
G) C) and D)

Question 11

What is the major limitation of the dried preparation of the insecticide produced by Bacillus thuringiensis?

Accepted Answer

A)   It has not been approved for use in the United States.
B)   It degrades so easily that it has to be applied repeatedly.
C)   It is not very toxic.
D)   It is new and untested.
E)   None of the aboveF) B) and E)
G) A) and E)

Question 12

Which of the following could not be used to test whether expression of a particular gene is necessary for a particular biological function?

Accepted Answer

A)   RNAi
B)   Knockout technology
C)   Antisense
D)   Mutant tRNA
E)   Transposon mutagenesisF) A) and B)
G) D) and E)

Question 13

_______ are the smallest general class of vectors.

Accepted Answer

The answer of _______ are the smallest general class of...

Question 14

Restriction enzymes recognize and cut at DNA sequences that are _______ (i.e., that read the same way in both directions).

Accepted Answer

palindromi...

Question 15

DNA chip technologies can be used to

Accepted Answer

A)   predict who will get cancer.
B)   show transcriptional patterns in an organism during different times of development.
C)   clone DNA.
D)   make transgenic plants.
E)   inhibit transcription of disease genes.F) D) and E)
G) All of the above

Question 16

The RNA in RNAi (RNA interference)  is

Accepted Answer

A)   single stranded.
B)   not made in vivo.
C)   relatively stable in cells.
D)   capable of binding to specific mRNAs.
E)   Both c and dF) A) and E)
G) C) and D)

Question 17

What information would you need to design a synthetic gene for a protein to be expressed (translated)  in yeast?

Accepted Answer

A)   Primary structure of the protein and the genetic code
B)   Primary structure and secondary structure of the protein and the genetic code
C)   Primary structure of the protein, the genetic code, promoter sequence, and codon usage
D)   Secondary structure of the protein, the genetic code, and promoter sequence
E)   Primary structure of the protein and promoter sequenceF) B) and D)
G) All of the above

Question 18

Recombinant DNA (rDNA)  technology is least applicable to which of the following approaches?

Accepted Answer

A)   The analysis of traits determined by multiple genes
B)   Overexpression of a particular gene
C)   Silencing a particular gene
D)   Knocking out a particular gene
E)   Targeting a protein to the nucleusF) D) and E)
G) A) and C)

Question 19

Why is the use of RNAi as a means for blocking RNA translation preferred over the use of antisense RNA?

Accepted Answer

A)   RNAi can be used with DNA chips.
B)   Only RNAi can be used to test cause-and-effect relationships.
C)   RNAi is more stable than antisense RNA.
D)   RNAi is single-stranded and thus easier to produce.
E)   None of the aboveF) C) and D)
G) B) and D)

Question 20

Recombinant DNA technology requires not only getting the DNA into the cell, but also getting it to replicate appropriately. In this process, the specificity of which of the following enzymes can present a challenge?

Accepted Answer

A)   DNA ligase
B)   DNA polymerase
C)   RNA polymerase
D)   Reverse transcriptase
E)   ReplicaseF) C) and E)
G) A) and E)

Exam 18: Recombinent Dna and Biotechnology

What is the major limitation of the dried preparation of the insecticide produced by Bacillus thuringiensis?

Correct AnswerverifiedShow Answer

Libraries constructed from only those genes that are expressed in a given tissue are known as _______ libraries.

Correct AnswerverifiedShow Answer

Restriction enzymes recognize and cut at DNA sequences that are _______ (i.e., that read the same way in both directions).

Correct Answerverifiedpalindromi...View AnswerShow Answer

The DNA from the genome of an organism is chopped into numerous fragments, which then are inserted into vectors that are taken up by host cells. The genetic information that is present in the resulting colonies is called a genomic

Correct AnswerverifiedShow Answer

An enhancer would most likely be used for

Correct AnswerverifiedShow Answer

Complementary base pairing is important for

Correct AnswerverifiedShow Answer

DNA chip technologies can be used to

Correct AnswerverifiedShow Answer

Which of the following would not be considered a form of biotechnology?

Correct AnswerverifiedShow Answer

_______ are the smallest general class of vectors.

Correct AnswerverifiedShow Answer

Why is the use of RNAi as a means for blocking RNA translation preferred over the use of antisense RNA?

Correct AnswerverifiedShow Answer

What information would you need to design a synthetic gene for a protein to be expressed (translated) in yeast?

Correct AnswerverifiedShow Answer

The joining of DNAs from two or more genetic sources DNA is called

Correct AnswerverifiedShow Answer

Recombinant DNA technology requires not only getting the DNA into the cell, but also getting it to replicate appropriately. In this process, the specificity of which of the following enzymes can present a challenge?

Correct AnswerverifiedShow Answer

_______ would be added to an expression vector if the researchers wanted to direct the gene product to the nucleus after its production.

Correct AnswerverifiedShow Answer

A biologist would most likely use the technique of electroporation to

Correct AnswerverifiedShow Answer

The production of a hormone in milk from a transgenic cow is an example of _______.

Correct AnswerverifiedShow Answer

Which of the following could not be used to test whether expression of a particular gene is necessary for a particular biological function?

Correct AnswerverifiedShow Answer

The RNA in RNAi (RNA interference) is

Correct AnswerverifiedShow Answer

Vectors include

Correct AnswerverifiedShow Answer

Recombinant DNA (rDNA) technology is least applicable to which of the following approaches?

Correct AnswerverifiedShow Answer

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