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Which of the following best describes the principle of the Kleihauer-Betke test?


A) Fetal hemoglobin is resistant to acid elution and remains in the cell to stain pink,whereas adult cells appear as ghost cells.
B) Adult hemoglobin is resistant to acid elution and remains in the cell to stain pink,whereas fetal cells appear as ghost cells.
C) D-positive cells from the fetus form rosettes around the IgG-coated mother's cells.
D) Indicator cells form rosettes around the fetal D-positive cells.

E) None of the above
F) All of the above

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Which elution method is ideal in the investigation of ABO hemolytic disease of the fetus and newborn?


A) Glycine-acid
B) Freeze-thaw
C) Xylene
D) Chloroform

E) A) and C)
F) B) and D)

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In which type of hemolytic disease of the fetus and newborn is the first-born affected?


A) ABO
B) D
C) E
D) M

E) All of the above
F) B) and C)

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During a first-trimester prenatal examination,it was determined that a pregnant woman was group A,D-negative with an anti-D titer of 8.What is the most likely course of action?


A) The anti-D is probably from prenatal Rh immune globulin and should be given another dose at birth.
B) Repeat the titer in 4 weeks to determine if significant if a rise in titer is detected.
C) The pregnancy is considered high risk,and the mother should be followed up immediately with amniocentesis or percutaneous umbilical cord testing.
D) None of the above are correct.

E) All of the above
F) B) and D)

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Why is reverse grouping omitted in the neonatal period for ABO testing?


A) The maternal antibody is identical to that of the newborn.
B) Newborns do not produce their own antibody until about 4 months.
C) The newborn's antibody is the same as the paternal antibody.
D) None of the above is correct.

E) B) and C)
F) A) and B)

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Select the most common antibody specificity associated with hemolytic disease of the fetus and newborn.


A) Anti-A,B
B) Anti-C
C) Anti-D
D) Anti-K

E) B) and D)
F) A) and D)

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The Kleihauer Betke test was performed using a 1-hour postdelivery maternal blood sample.Results: 10 fetal cells/1000 cells counted It is the policy to add 1 vial of Rh immune globulin to the calculated dose when the estimated volume of the hemorrhage exceeds 20 mL of whole blood.Calculate the number of vials of Rh immune globulin that would be indicated under these circumstances.


A) 2
B) 3
C) 4
D) 5

E) B) and C)
F) A) and D)

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Amniotic fluid analysis showed a marked increase into zone III of the Liley graph.Lecithin-sphingomyelin ratios indicated that the fetal lungs were not mature.Select the most appropriate decision regarding medical intervention.


A) No immediate need for intervention
B) An intrauterine transfusion
C) Delivery by cesarean section
D) None of the above

E) A) and B)
F) A) and C)

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A .3% fetomaternal bleed was determined by a Kleihauer Betke stain of postpartum blood.What is the estimated volume of the fetomaternal bleed expressed as whole blood?


A) 10 mL
B) 15 mL
C) 20 mL
D) 25 mL

E) B) and C)
F) None of the above

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To prevent graft-versus-host disease,red blood cells prepared for intrauterine transfusions should be:


A) frozen and deglycerolized.
B) saline washed.
C) ABO and D-compatible with the mother.
D) irradiated.

E) None of the above
F) A) and B)

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